Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia

An urban park is potentially a source of vector-borne disease transmission due to it being natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims screen the occurrence knockdown-resistance (kdr) mutant alleles (V1016G F1534C) in populations collected from parks Surabaya, Indonesia. Cross sectional was conducted July 2019. A total 28 ovitraps were installed seven parks, having four each park. In total, 1,662 eggs collected, only 187 emerged into adult mosquitoes, consisting 97 Aedes (Stegomyia) aegypti 90 albopictus. All-female mosquitoes (n=55) tested using allele-specific polymerase chain reaction assay (AS-PCR) detect voltage gated sodium channel (VGSC) gene mutations. This found no mutations Valine Glysine mutation point 1016 (V1016G) Phenylalanine Cysteine 1534 (F1534C) both two species. All samples have wild type genotype kdr (V1016V F1534F). Data analysed R Studio 1.4 Version by Genetics package. Results showed that frequency resistant (G1016 C1534) zero, susceptible allele 1 (V1016 F1534). Insecticide bioassay could not be established limited number so insecticide resistance status determined. However, can used as preliminary monitoring for vector control program.